Composite

Part:BBa_K1493560

Designed by: Michiel Herpers   Group: iGEM14_Wageningen_UR   (2014-10-06)

pRHA+cIλ+pcI/tet+GFP


L-Rhamnose inducable promoter witht the cI lambda represor combined with a PcI/tet with GFP.

When rhamnose is present the cI lambda repressor is expressed, repressing the pcI/Tet promoter and so inhibiting the expression of GFP.

This BioBrick is used by [http://2014.igem.org/Team:Wageningen_UR BananaGuard] to characterize the pcI/tet promoter with the [http://2014.igem.org/Team:Wageningen_UR/project/characterization rhamnose mediated characterization].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1811


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Parameters
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